Characterization of Cysteine Dioxygenase Variants by Trapped Ion Mobility
Mass spectrometry is extensively used for the characterization of intact proteins’ molecular weight. Separation in gas phase by trapped ion mobility measurements can facilitate these studies by reducing the sample complexity. Understanding the mechanism of the Cys-Tyr cross-link biogenesis in human CDO is difficult due to closely related isoforms in the starting material for studying cofactor biogenesis. For this reason, a method using trapped ion mobility separation (TIMS) was developed to separate crosslinked and uncrosslinked variants.
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