Characterization of IgG Monomers & Their Aggregates
In this application note we characterize two antibody samples using column calibration and multi-detection SEC. Here we show the outcomes of 2 Characterization of IgG monomers & their aggregates both analyses and demonstrate how multi-detection SEC - a technique that combines the resolving power of chromatography with the revealing power of light scattering detectors and a viscometer. In a multi-detection SEC system, the light scattering detectors measure absolute molecular weight (as opposed to a column calibration system that can only provide a relative measurement) and the differential viscometer provides information about the molecular structure of the protein being analyzed. This wider range of accurate data provides a more complete characterization of the protein mixtures under study is obtained.