Characterization of Protein Aggregates in Suspension and on a Filter Membrane by Morphologically-Directed Raman Spectroscopy

The Morphologi G3-ID, a hybrid optical microscope-Raman spectrometer from Malvern Instruments, provides the imaging power of a high-resolution optical microscope with the chemical identification capabilities of a benchtop Raman spectrometer, and is completely automatable. 

This application note explores the use of the Morphologi G3-ID for Morphologically-Directed Raman spectroscopy (MDRS) to identify and analyze contaminants including protein aggregates present in a stressed sample of lysozyme, and to compare particles held in suspension within a thin path wet cell with those collected on a filter membrane.