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Application Note

Characterization of Protein-Protein Interactions using Triple Detection Size Exclusion Chromatography (SEC-TD)

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Application Note

Characterization of Protein-Protein Interactions using Triple Detection Size Exclusion Chromatography (SEC-TD)

The ability of proteins to form ordered, structured complexes is central to their biological function. Such complexes, which can be homo-oligomers or heterooligomers, can control different attributes such as activity, stability and localization. Understanding how these complexes form, and the controlling mechanism behind them, is fundamental to our knowledge of protein signaling pathways and the physiological responses they control. Of particular interest in this field, is the assembly of virulence factors complexes from pathogenic microbes. These factors are secreted by such micro-organisms to facilitate infection, survival and/or colonization of the host and are therefore crucial for the onset of disease. The ability to target and inhibit these virulence factors could lead to the development of a new generation of anti-microbial therapies. A prerequisite is to understand the mechanisms leading to the formation of hetero- and homo-oligomers. In order to characterize the protein-protein interactions of selected microbial virulence factors, advanced light scattering detectors, combined with liquid chromatography methods, were employed.

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