Determining the Fluorescence Limit of Detection for a Given Flourophore

This short study aims to look at the sensitivity of NTA in terms of measuring fluorescently labelled particles using standard materials with known affinity strengths. It makes a number of assumptions (gold particle surface area, biotin coverage, complete streptavidin binding, homogeneous sample incubations), but demonstrates that with an equivalence of 5 fluorescent streptavidin molecules per gold nanoparticle some gold particles are detected as being fluorescently labelled.