Determining the Fluorescence Limit of Detection for a Given Flourophore
Application Note Jun 04, 2015
Malvern Instruments Limited
This short study aims to look at the sensitivity of NTA in terms of measuring fluorescently labelled particles using standard materials with known affinity strengths. It makes a number of assumptions (gold particle surface area, biotin coverage, complete streptavidin binding, homogeneous sample incubations), but demonstrates that with an equivalence of 5 fluorescent streptavidin molecules per gold nanoparticle some gold particles are detected as being fluorescently labelled.