Gas Chromatographic Analysis of Polyunsaturated FAME in Biodiesel Made From Algae and Marine Oils

The GC analysis of four common polyunsaturated fatty acid methyl esters (PUFA FAMEs) in algal biodiesel is described using method EN 15779. An Agilent 7890A GC system was configured and calibrated according to the procedure outlined in the method. Two Samples of B100 biodiesel made from algae oil were each prepared in duplicate and analyzed according to the conditions set forth in the method. In each sample, the four PUFA FAMEs were chromatographically separated and qualified. The analysis precision was calculated and shown to exceed the specifications of the EN 15779 methods.