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HPLC-DAD Analysis of Nucleotides Using a Fully Inert Flowpath

The chromatographic analysis of phosphorylated species is highly challenging. Phosphorylated compounds such as nucleotides are known to interact with stainless steel components leading to recovery issues in HPLC and LC/MS.

Download this application note to discover:

  • How to analyze nucleotides using the Agilent 1260 Infinity II Bio-Inert LC System
  • How the fully inert flowpath, governed by the setup, leads to substantially improved recoveries for challenging phosphorylated solutes
  • How the PEEK-lined InfinityLab Poroshell 120 HILIC-Z column can improve your peak shape and quality of your chromatogram