Identification of Human Hemoglobin Protein Variants Using Electrospray Ionization-Electron Transfer Dissociation Mass Spectrometry

In this application note, the doubly-charged tryptic peptide from a variant of low abundance occurred at the same m/z value as a singly-charged interfering ion. Here, we demonstrate fragmentation of the triply-charged tryptic peptide ions using electron transfer dissociation, (as opposed to collision induced dissociation of the triply-charged ions) for sequence information. This technique is shown for the identification of an alpha-chain variant having normal clinical presentation, namely Hb Riccarton.

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