Investigating the Effects of Excipients on the Earliest Stages of Aggregation - Aggregation Inhibition by Arginine
Application Note Oct 25, 2016
Malvern Instruments Limited
This application note describes the characterization of a model protein (lysozyme) in the presence and absence of arginine using the array of techniques provided by the Zetasizer Nano ZSP. SLS is used to assess the temperature at which lysozyme aggregation begins, with temperature control provided by the instrument's peltier elements. DLS is used to assess the various particle sizes present in the two formulations at different temperatures, whilst measurements of zeta potential are used to assess the effect of arginine on the charge state of the protein.