Novel Chemical Standards Kits Enable Facile Lipid Quantitation
Application Note Jul 11, 2016
A novel lipidomics platform was developed that includes simplified sample preparation, automated methods, and streamlined data processing techniques, for facile, quantitative lipid analysis1. Unique internal standards, tuning kits and system suitability kits were designed to overcome a gap in the lipidomics field. Traditionally a single internal standard per class had been used as a strategy in quantitating lipid molecular species, however the diversity of fatty acid chain lengths and degrees of unsaturation for molecular species that provide differential fragmentation efficiency were unaccounted for, which fundamentally impacts quantitation. The LipidyzerTM platform internal standards, which contain over 50 labeled molecular species across thirteen classes, neutralize this quantitative bias and allow for more accurate measurement.
In addition to the labelled internal standards, the platform is equipped with additional kits which allow a user to assess the reproducibility and sensitivity of their system before running samples. The System Suitability kit enables the user to assess the sensitivity of the assay and the reproducibility (robustness) of the platform. The SelexION® Technology Tuning kit allows the automated optimization of the differential mobility spectrometry (DMS) cell which aids in definitive lipid identification2. Finally, the QC Spike Standards kit, which contains unlabeled molecular lipid species, can be added to the QC control plasma at a known concentration and monitored throughout the analysis as a QC sample.