Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules

In the process of candidate selection, data regarding  the aggregation propensity of antibody molecules in various buffer conditions would aid in the prediction of problems that would potentially be encountered during manufacturing and shelf-life stability. Shaking stress is a convenient method to screen and compare the robustness of antibodies in different buffer conditions in manufacturing  and serves to mimic the effect of stress at air-liquid interfaces.

A higher throughput (96 well plate format) screening method is required which would allow continuous real time measurement of aggregation within a convenient time window of analysis.

This application note details how the FLUOstar Omega microplate reader from BMG LABTECH equipped with shaking options (allowing for linear, orbital and double orbital continuous shaking) with both absorbance and fluorescence detection set up in the kinetic mode was utilised to explore a more efficient method for measuring aggregation propensity.