Quantitative Determination of Caffeine in Beverages and soft Drinks Using UV Wavelength Spectroscopy

Numerous studies, employing a wide variety of methods, have been carried out to determine the typical caffeine content of commonly consumed beverages. HPLC is the method of choice in many analytical studies. However HPLC is an expensive and resource-hungry technique that is not typically found in the scientific teaching labs of schools and colleges.

This application note details how UV spectroscopy can be used to analyse and quantify the caffeine content of some common beverages and soda drinks.