Reach New Levels of Metabolite Characterization

Early ADME studies are used to determine if a drug candidate is prone to metabolic breakdown or oxidation/conjugation. Glucuronide conjugation is difficult to characterize using MS/MS alone, therefore fragmentation is also required to obtain structural information. 

Electron-activated dissociation (EAD) is an orthogonal fragmentation technique that can generate fragments of glucuronide conjugates that retain the glucuronic acid group, allowing for a confident assessment of the conjugation site. 

Download this app note to:

• Obtain richer fragmentation patterns
• Generate diagnostic spectral peaks for labile fragments
• Confidently differentiate between conjugation sites