Replicating an HPLC Method for Water-Soluble Vitamins on the Alliance™ iS HPLC System

Vitamin analysis is performed regularly in a wide array of laboratories. For dietary supplement laboratories, it is useful to test samples throughout the production process to ensure that ingredients and finished products have the expected concentration of desired vitamins. In the United States, 21 CFR 101.9(g)(4) indicates that added Class I ingredients must be at least 100% of the declared value on the label, with consideration of analytical method variation.2 Products with labels that do not meet this requirement are considered misbranded, which further justifies the significance of analytical testing.
When performing vitamin analysis, sample preparation can be difficult as matrix interferences may be present in some multivitamin formulations. At times, this can be mitigated with additional sample dilutions, solid phase extraction techniques or liquid-liquid extractions. For more complex matrices, additional sample cleanup or multiple extractions may be required.
In addition to the different analytical challenges that vitamins pose naturally, within a typical analytical laboratory, there are a variety of HPLC systems that can be used for analysis. Furthermore, as labs update their instrumentation, there may be a need to take advantage of modern technologies. These factors make it important to be able to migrate methods across different systems, with each system producing the same quantitative results for a given sample.
This work will demonstrate the migration of a multivitamin analysis from a legacy HPLC system, specifically the Alliance e2695 HPLC System, to an Alliance iS HPLC System. Comparable quantitative results between the two systems will be shown.