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Scaling and Migration of a Hydrophilic Interaction Liquid Chromatography (HILIC) Method for Related Compounds of Ribavirin to Modern HPLC Systems

Scaling and Migration of a Hydrophilic Interaction Liquid Chromatography (HILIC) Method for Related Compounds of Ribavirin to Modern HPLC Systems
Credit: iStock

Achieving identical chromatographic separation and equivalent results are critical for a successful method scaling across different particle size columns and migration between LC systems. In this work, a HILIC method for the analysis of active pharmaceutical ingredient (API), ribavirin, and related compounds was scaled from a 1.7 μm (2.1 x 50 mm) column to 2.5 μm (3.0 x 75 mm), and 3.5 μm (4.6 x 100 mm) columns with equivalent chemistry.
The method was then migrated from an ACQUITY™ UPLC™ H-Class PLUS System to ACQUITY Arc™, Arc HPLC, and Alliance™ iS HPLC systems. Equivalent performance was demonstrated by assessing chromatographic separation, system suitability, and assay results generated by the method on the different systems.