Tools to Assist in Moving Established Methods from an Originator HPLC System to an Alliance™ iS HPLC System

Transferring a method from a legacy HPLC instrument to a new HPLC system can be challenging. Differences in system characteristics, including dwell volume and or gradient delay, can produce retention time shifts when transferring a gradient method between two HPLC Systems. In addition, creating a new instrument method and copying settings from one instrument method to another can potentially introduce transcription errors. Using a set of embedded software tools can reduce error from these manual processes. In this work, two software tools will be used to reduce error in moving a method to the Alliance iS HPLC System. The Intelligent Method Translator App (iMTA) will demonstrate the ability to transcribe critical method parameters from an originator HPLC System to the Alliance iS HPLC System. In addition, Gradient Smart Start will be used to adjust the start of the gradient with respect to injection time (i.e. adjusting the duration of the gradient delay), eliminating the need to make changes to the gradient table to replicate retention times when moving between systems with different gradient delay volumes. This example will demonstrate the ease and added compliance provided in using these embedded software tools in the replication or transfer of an existing method to the Alliance iS HPLC System.