ddPCR Microsatellite Instability (MSI) Kit

ddPCR Microsatellite Instability (MSI) Kit

The ddPCR Microsatellite Instability (MSI) Kit provides sensitive, reproducible, qualitative results for the detection of deletions and insertions in five mononucleotide microsatellite markers in plasma or tumor tissue samples.

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Overview

The ddPCR Microsatellite Instability (MSI) Kit provides sensitive, reproducible, qualitative results for the detection of deletions and insertions in five mononucleotide microsatellite markers in plasma or tumor tissue samples.

Further, the kit allows for the detection of deletions and insertions in microsatellite markers BAT25, BAT26, NR21, NR24, and Mono27 in plasma or tumor tissue, including formalin-fixed paraffin-embedded (FFPE) tissue, from colorectal cancer (CRC) patients and other potentially MSI-relevant cancers. The kit consists of three assays: Assay 1 (BAT25 and BAT26), Assay 2 (NR21 and NR24), and Assay 3 (Mono27) as well as positive and no template controls, and enough reagents for 200 reactions. Additionally, an automated software tool is available for sample analysis.

Features and Benefits

  • Evaluate MSI markers with both FFPE and plasma samples
  • No need for normal tissue samples
  • Improved workflow and cost efficiency over current methods
  • Automated data analysis with user-defined parameters
  • Excellent concordance with gold standard IHC and PCR-CE methods

Applications and Uses

Detecting microsatellite instability in tissue and plasma samples from colorectal cancer patients and other potentially MSI-relevant cancers.

Note: For research use only.

Product Content

Material Number Component Quantity
12005915 ddPCR Multiplex Supermix 2
16009019 ddPCR MSI Assay 1 (BAT25/BAT26) 1
16009020 ddPCR MSI Assay 2 (NR21/NR24) 1
16009029 ddPCR MSI Assay 3 (Mono27) 1
16009021 ddPCR Positive Control 1
16009039 or 12017702 Uracil-DNA Glycosylase (UDG) 1
8006877 Nuclease-Free Water 2

Configuration Options

Bundles or Different Component Sets Catalog Number
QX200 Droplet Reader 1864003
Automated Droplet Generator 1864101
QX200 Droplet Generator 1864002
PTC Tempo Thermal Cycler 12015392
C1000 Touch Thermal Cycler 1851197
PX1 PCR Plate Sealer 1814000

Related Products

  • BRAF V600 ddPCR Multiplex Mutation Screening Kit
  • KRAS ddPCR Multiplex Mutation Screening Kits
  • NRAS ddPCR Multiplex Mutation Screening Kits
  • EGFR Exon19 Deletions ddPCR Multiplex Mutation Kit

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ddPCR Microsatellite Instability (MSI) Kit
12015172

200 x 20 ul reactions, includes ddPCR MSI Assay 1 (BAT25/26), MSI Assay 2 (NR21/24), MSI Assay 3 (Mon27), ddPCR Multiplex Supermix, Uracil-DNA Glycosylase (UDG), positive control, and nuclease-free water for screening mutations in 5 MSI markers using the QX200 or QX200 AutoDG ddPCR Systems

Downloads

Microsatellite Instability (MSI) Kit, Software, FFPE APF, Version 1.0
MSI10_FFPE

Microsatellite Instability (MSI) Kit, Software, FFPE APF, Version 1.0

Microsatellite Instability (MSI) Kit Software, FFPE Quality Rules APF, Version 1.0
MSI10_FFPE_QR

Microsatellite Instability (MSI) Kit Software, FFPE Quality Rules APF, Version 1.0

Microsatellite Instability (MSI) Kit Software, Plasma APF, Version 1.0
MSI10_Plasma

Microsatellite Instability (MSI) Kit Software, Plasma APF, Version 1.0

Microsatellite Instability (MSI) Kit Software, Plasma Quality Rules APF, Version 1.0
MSI10_Plasma_QR

Microsatellite Instability (MSI) Kit Plasma Quality Rules APF Software, Version 1.0

Documents

Number Description Options
10000142159
ddPCR Microsatellite Instability (MSI) Kit User Guide
3532
Microsatellite Instability (MSI) Kit Flyer