In flow cytometry, some of the fluorescence will be collected in the detection channel of interest, but some of the fluorescence will overlap with other channels. Compensation is used to remove the unwanted light signals from all but the desired detector. In traditional flow cytometers, the voltage for all of the detectors must be set and fixed during the collection of the single–color compensation controls and the samples.
Download this whitepaper to discover a platform that:
- Can circumvent the requirement for constant gain settings
- Allows user to adjust the measurement of one or more of the signals during the workflow
- Automatically recalculates spillover values in real time as the gains are adjusted
