Successfully mastering agarose gel electrophoresis has become an essential skill for most molecular biologists.
Done well, the technique can help us diagnose disease, separate desirable from undesirable fragments for further assays and confirm if experiments have been successful. However, there are many pitfalls that can spoil your day, leading to gel failure, sample loss, insufficient separation or uninterpretable results.
Download this guide to explore key considerations to remember when:
- Selecting the concentration of agarose required to give optimal separation
- Pouring and running your gel
- Maintaining your equipment