qPCR Assays: Tips, Tricks and Best Practice
A successful qPCR assay requires efficient and specific amplification of the product. Primers and the target sequence can both impact amplification efficiency and specificity and thus the accuracy of the assay.
Download this guide to discover top tips for:
- Designing primers and hydrolysis probes
- Increasing reproducibility
- Minimizing contamination
- Avoiding potential real-time PCR pitfalls