Advancing AAV Characterization Through Mass Photometry

Accurate single-particle AAV capsid measurements across a broad dynamic range Intuitive instruments and software take you from sample to result in less than 5 minutes Mass photometry and the SamuxMP portfolio Rapid analysis for AAV development and GMP manufacturing Software modules enable compliance in GMP-regulated environments Mass photometry provides comprehensive AAV purity analytics Mass photometry is a fast, powerful AAV analytics tool that can be used for frequent testing during process development. Its ability to provide single-particle resolution measurements allows for quantitative analysis of empty, partial and full AAV capsids as well as determination of cargo genomic length. Empty, partial and full analysis Cargo genomic length AUC qPCR/ ELISA TEM Mass photometry Capsid fill ratio (empty, full, partials)   (Does not resolve partials)  (Does not resolve partials)  Turnaround time Sample volume Ease of use Serotype agnostic     Cost per sample $ $ $ $ $ $ $ $ $ Comparing tools for AAV downstream analytics Analytical technologies commonly used in AAV downstream analytics include analytical ultracentrifugation (AUC), quantitative PCR with ELISA (qPCR/ELISA), transmission electron microscopy (TEM) and mass photometry. They have different capabilities and limitations, reflecting the differences in the underlying technologies. Empty:full purity analysis in AAV production Measuring purity attributes such as empty, full, and partial capsids is an informative parameter across many steps along the AAV production workflow, especially when progressing into and through downstream processing. Upstream Downstream Fill / finish Clarification Concentration Capture Polish Concentration/ buffer exchange Empty:full analysis Empty:full analysis Empty:full analysis Limitations of existing methods for assessing AAV purity in downstream processing It can be difficult to assess CQAs efficiently and frequently throughout the production workflow due to limitations of existing methodologies. Large sample volume requirements Low assay throughput Low resolution and sensitivity Time-consuming High cost per sample Issues with accuracy and reproducibility Key analytical attributes in AAV production Developing an efficient and effective workflow to produce adeno-associated virus (AAV) therapeutics requires the ability to monitor a range of critical quality attributes (CQAs). Replication competence, bioburden, endotoxin, sterility, mycoplasma, mycobacteria Safety Capsid / serotype and genome integrity Identity Infectious and functional titer Potency Viral and genome titer Content pH, osmolality, appearance, visible particles, sub-visible particles Physical Process and product impurities including assessment of cargo genomic length, and empty, partial and full capsid analysis Purity CQAs Unit 9, Trade City, Sandy Lane West, Oxford OX4 6FF, United Kingdom ©2025 Refeyn Ltd Refeyn and Samux are trademarks of Refeyn Ltd. refeyn.com Refeyn Refeyn Learn more at refeyn.com/aav-vector-analytics Revolutionizing AAV analytics with mass photometry