Improve Separation With Orthogonal LC

1290 Infinithy III 1290 Infinithy III 1290 Infinithy III 1290 Infinithy III 1290 Infinithy III 309/12 bar Pump 420 2.521 mL/min Pump 20 °C Column Comp. 20 °C Sequence/Sample Method Sample Location (Seq. Table) 02:46 13:30 Edit Unlock Pump A 60% B 20% D 10% C 10% 1 0.8 0.4 0.2 0 0.6 01:00 01:30 02:00 02:30 03:00 03:30 Signal A (mAU) Signal B (mAU) Multiple heart-cutting two dimensional liquid chromatography mass spectrometry: Towards real time determination of related impurities of bio-pharmaceuticals in salt based separation methods Click for article Conquer Complexity with Orthogonal LC Technologies Why orthogonal separation strategies matter Traditional chromatography methods are often used for assay and purity analysis of APIs and finished drug products. These methods may fall short when: - Structurally similar compounds (e.g. isomers) co-elute with the main component or each other. - Highly complex samples/sample matrices must be evaluated. - The source of impurity (e.g. sample versus external contamination) must be identified. - Selectivity changes during method development. - High-resolution, reproducible, and comprehensive impurity profiling is required to measure critical quality attributes or meet tight regulatory requirements. Orthogonal techniques like SFC and 2D-LC offer distinct separation mechanisms that complement traditional LC. By combining their independent selectivities, you reduce bias, improve accuracy, and better resolve complex pharmaceutical samples. Agilent 1290 Infinity III 2D-LC System - Advanced separations: Analyze complex mixtures with flexible 2D-LC modes, including both heart-cutting and comprehensive workflows. - Smart software: Simplify method setup and maximize sample insights with powerful data analysis and reporting. - High performance: Achieve fast, sensitive UHPLC results with robust hardware and the Agilent InfinityLab 2D-LC ASM Valve. - Scalable and biocompatible: Upgrade from 1D to 2D-LC and handle challenging samples with biocompatible flow paths. - Simplified operation: Accelerate your start with Agilent 2D‑LC by combining superior usability, an Easy Start Service, and customizable application examples. Let Agilent experts help you find the best orthogonal analysis solutions for your separation tasks Want to know more? Agilent 1260 Infinity III SFC and Agilent 1260 Infinity III SFC/UHPLC Hybrid Systems - Faster results: Supercritical CO2 enables rapid separations and higher throughput. - Greener, cost-effective: CO2 is ecofriendly, recyclable, and reduces solvent use and waste. - Chiral and polar expertise: Ideal for separating chiral and moderately polar compounds. - Hybrid flexibility: Switch easily between SFC and UHPLC for maximum versatility. - Simplified automation: Integrated software streamlines complex workflows and reduces manual steps. Sucessful use of 2D-LC stands and falls with dedicated software Elevate your complex separations Are you sure? Improve selectivity and resolution, avoiding surprises and regulatory issues by using full orthogonality to prove purity. Replace complex workflows Need to accelerate your workflow? Achieve higher sample throughput and faster separations, streamlining your processes. Become more sustainable and efficient Want better ROI? Save money and reduce solvent use. Support greener practices and improve sustainability. " We believe that Agilent’s multiple heart-cutting 2D-LC has the potential to fundamentally change how impurities are characterized and purity methods are developed in pharma." — Global Research Team, Novo Nordisk Learn more at:. www.agilent.com DE-012798 This information is subject to change without notice. © Agilent Technologies, Inc. 2026 Published in the USA, February 27, 2026 5994-9016EN Enhance your analytical capabilities, improve selectivity and resolution, and achieve more reliable results by integrating SFC and 2D-LC systems into your pharmaceutical research. Sample including molecules varying in size and charge Separation by size Selectivity α1 α2 Combining 1D α1/2D α2 Separation by charge Separation by size Separation by charge Universal tools to overcome your greatest chromatographic challenges Pharma scientists agree – Agilent orthogonal separation strategies deliver results - Leverage a powerful method editor with unique features - Refine 1D methods easily by selecting peaks for 2D analysis - Set up 2D gradients and optimize them for peak capacity and speed - Automatically optimize run time and peak‑storage capacity - Configure sampling volumes flexibly, create one or multiple cuts per peak, and retain 1D resolution - Analyze unknown samples using peak‑based and dynamic peak‑parking workflows - Perform simple yet powerful qualitative and quantitative data analysis—including UV and MS—using 2D‑LC Software for OpenLab CDS Learn more Click for 2D-LC Next steps Go to our store Click for SFC Minimize the guess work with our column selection guides for: 2D-LC – HPLC and LC Column Selector SFC – Extend Your Application Reach with Achiral and Chiral SFC Columns Not sure how to begin? Fill out this form or contact your local Agilent representative today for more details. Ready to add orthogonal techniques to your workflow? Buy online today: www.agilent.com/chem/store Explore solutions for small molecule drug characterization and purity analysis: Small Molecule Drug Characterization and Purity Analysis Explore solutions 5 10 15 20 min 0 50 100 mAU SFC HPLC Up to 10x times faster than HPLC UHPLC SFC HPLC SFC 0 % 20 % 40 % 60 % 80 % 100 %