3D Images Show How Sperm Binds to the Egg Surface
Crystal structure of the complex between a domain repeat of egg coat protein VERL (dark pink) and cognate sperm protein lysin (blue). Credit: Isha Raj and Luca Jovine.
Researchers at Karolinska Institutet in Sweden have obtained the first 3D snapshots of a sperm protein attached to a complementary egg coat protein at the beginning of fertilisation. The study, which reveals a common egg protein architecture that is involved in the interaction with sperm in both mollusc and mammal, is published in Cell.
By transmitting the genetic information to the next generation and marking the beginning of a new life, the encounter between female and male gametes at fertilisation is one of the most fundamental processes in biology. Although egg and sperm were first observed centuries ago, how sperm recognises the coat of the egg and penetrates it has remained unknown.
Using X-ray crystallographic data collected at the European Synchrotron Radiation Facility (ESRF), Luca Jovine's research team at Karolinska Institutet first visualised the sperm-interacting regions of two egg coat proteins, ZP2 in mammals (including humans) and VERL in the marine mollusc abalone (a classic model system of invertebrate fertilisation). Both of these molecules contain repeated sequences that play a key role in gamete recognition.
“Mammals and molluscs are thought to be separated by 600 million years of evolution, and their sperm receptor proteins are almost completely different in sequence. However, comparison of the structures conclusively demonstrates that ZP2 and VERL repeats share a common 3D architecture”, says Luca Jovine, Professor of Structural Biology at the Department of Biosciences and Nutrition and the Center for Innovative Medicine at Karolinska Institutet.
Subsequently, the research group determined crystal structures of different VERL repeats bound to lysin, the counterpart protein of VERL on abalone sperm. This gave an unprecedented view of how gametes recognise each other in a species-specific way at the beginning of fertilisation.
“Abalone was our system of choice for this investigation, as it is one of the few organisms where cognate egg coat and sperm proteins are known. Moreover, different species of abalone spawn in the open sea but, despite overlapping habitats and breeding seasons, hybrids rarely occur”, says Professor Jovine.
The VERL-lysin complex structures also suggest how lysin opens a hole into the egg coat, allowing sperm to penetrate into the egg.
“Gamete recognition was first compared to a lock and key mechanism more than one hundred years ago. Our study provides the first example of how this is achieved at the very beginning of fertilisation, concludes Luca Jovine.
This article has been republished from materials provided by Kaolinska Instituet. Note: material may have been edited for length and content. For further information, please contact the cited source.
Jovine et al, ‘Structural Basis of Egg Coat-Sperm Recognition at Fertilization’, Cell 2017. DOI: 10.1016/j.cell.2017.05.033
Building Molecular Wires, One Atom at a TimeNews
Electronic devices are getting smaller and smaller. Early computers filled entire rooms. Today you can hold one in the palm of your hand. Now the field of molecular electronics is taking miniaturization to the next level. Researchers are creating electronic components so tiny they can’t be seen with the naked eye.READ MORE
Longevity Protein Reveals its SecretsNews
In a recent study, researchers revealed the three-dimensional structure of a proteins linked to longevity and metabolism, beta-Klotho, illuminating its intricate mechanism and therapeutic potential. The study findings could have implications for therapies developed to treat a wide range of medical conditions, including diabetes, obesity, and certain cancers.READ MORE
Multidisciplinary Study Suggests New Strategy for Drug DiscoveryNews
A joint industry/academia study of a cancer target protein reveals unusual relation between binding site flexibility and drug-target lifetime. The results, , suggest a new strategy for drug discovery.READ MORE