We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Rectangle Image
News

An Adaptive Alignment Algorithm for Quality-Controlled Label-Free LC-MS

Rectangle Image
News

An Adaptive Alignment Algorithm for Quality-Controlled Label-Free LC-MS

Read time:
 

Abstract
Label-free quantification using precursor-based intensities is a versatile workflow for large-scale proteomics studies. The method requires extensive computational analysis and is therefore in need of robust quality control during the data mining stage. We present a new label-free data analysis workflow integrated into a multi-user software platform. A novel adaptive alignment algorithm has been developed to minimize the possible systematic bias introduced into the analysis. Parameters are estimated on the fly from the data at hand, producing a user-friendly analysis suite. Quality metrics are output in every step of the analysis as well as actively incorporated into the parameter estimation. We furthermore show the improvement of this system by comprehensive comparison to classical label-free analysis methodology as well as current state-of-the-art software.

The article is published online in Molecular & Cellular Proteomics and is free to access.


Advertisement