Bacterial Pac Man Molecule Snaps at Sugar
Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn, in cooperation with researchers at the University of York, have analysed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host.
The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system with which they can absorb the sugar compound. They use sialic acid, for instance, to produce energy, but also to escape the eagle eyes of the immune system. To do this, they incorporate the sugar into their cell surface. Thanks to this invisibility cloak made from substances produced naturally in the body, immune cells do not notice that the bacteria are intruders.
Many bacteria are surrounded by two membranes, which cover them like a thick skin. The absorption of sialic acid through the inner membrane often takes place via molecules that are known as TRAP transporters. They are comprised of three components. One of these, the P domain, moves freely and patrols between the two membranes. If it meets a sialic acid molecule, it grabs it and brings it to the inner membrane, where the two other components are firmly anchored. They then translocate the sugar compound into the bacteria cell.
“The atomic structure of the P domain was already known,” explains Dr. Gregor Hagelüken from the Institute for Physical and Theoretical Chemistry at the University of Bonn. “Together with our collaborator Dr. Gavin Thomas from the University of York, we wanted to analyse how exactly the P domain grabs the sialic acid. We have now been able to show that it lies in wait with its mouth open until it encounters a sugar molecule. At this moment, its jaws snap shut.” The P domain thus performs a rapid grabbing motion, which is somewhat reminiscent of the computer game character Pac Man.
The researchers used EPR spectroscopy in their work, a method with which the distances between parts of molecules can be measured precisely to a ten-millionth of a millimetre. In this way, they were able to understand the movement of the “Pac Man jaw”.
“To achieve this, we planted the blueprint for the P domain in harmless intestinal bacteria and grew them in a test tube,” explains Janin Glänzer, PhD student at the Institute. “We then cleaned the protein in an elaborate procedure and provided it with various quantities of sialic acid.”
To investigate the masticatory movement, the researchers fitted molecular labels to the “jaw” of the P domain, and then measured the precise distance between these markers. “We were able to show that the P domain can only exist in two forms, opened and closed,” says Martin Peter, who worked on this topic in his master thesis. “We were not able to identify any stable intermediate form. Without sialic acid, all P domains are found in an open state. The more sugar we provided, the more P domains were closed.”
Hagelüken hopes that the knowledge may help to develop new active ingredients against pathogens. “We can, for instance, attempt to prevent the masticatory movement, for example, using a molecular muzzle.”
Glaenzer, J., Peter, M. F., Thomas, G. H., & Hagelueken, G. (2017). PELDOR spectroscopy reveals Two defined states of a Sialic acid TRAP transporter SBP in solution. Biophysical Journal, 112(1), 109–120. doi:10.1016/j.bpj.2016.12.010
Please note: The content above may have been edited to ensure it is in keeping with Technology Networks’ style and length guidelines.
Quotient Sciences Acquires Pharmaterials, a UK-based Contract Development and Manufacturing OrganizationNews
Quotient Sciences, the drug development services organization, announces it has acquired Pharmaterials, a contract development and manufacturing organization (CDMO) based in Reading, U.K..READ MORE
Simple Water Test Could Prevent Crippling Bone DiseaseNews
A simple colour-changing test to detect fluoride in drinking water, could in the future prevent the crippling bone disease, skeletal fluorosis, in developing countries such as India and Tanzania.READ MORE
Allergy Amplifier Implicated in Asthma Also Intensifies Food AllergyNews
Scientist who previously reported that a small protein, aptly named histamine-releasing factor, played a pro-inflammatory role in asthma have now shown that it also serves as a “food allergy amplifier”. This work paves the way for blood tests to predict which patients will respond to allergy therapy, and strongly supports the idea that drugs designed to block HRF could prevent food allergy attacks.READ MORE