Background: Infant gut-associated bifidobacteria possess lacto-N-biosidase, which releases lacto-N-biose I (LNB) from human milk oligosaccharides.
Results: The crystal structures of lacto-N-biosidase complexed with LNB and LNB-thiazoline were determined.
Conclusion: The intermediate analogue complex allows the proposal of a conformational reaction coordinate.
Significance: The structures of a key enzyme in the colonization of human commensal bacteria provided its structural basis and insight into the development of inhibitors.
This article was published online in The Journal of Biological Chemistry and is free to access.