Discover the Mysteries of the Brain with Applied Biosystems iTRAQ Reagents
News Nov 24, 2005
Researchers at the Centre for Neurogenomics and Cognitive Research, Vrije Universiteit, in The Netherlands, have announced that they are using Applied Biosystems iTRAQ reagent kits to analyse proteins in brain synapses using tandem mass spectrometry (MS/MS) on the Applied Biosystems 4700 Proteomics Analyzer.
The team are characterising the dynamic changes taking place in the synapse during the changing conditions involved in memory creation, learning and behaviour.
Dr Ka Wan Li, Assistant Professor, explained, "There are about 2000 different proteins in the synapse and they play major roles in brain function and plasticity."
"Unfortunately, many of them are membrane proteins, which cannot be easily separated on 2D gels. Instead we solubilise, digest and label them with iTRAQ reagents, then analyse them on the 4700 Proteomics Analyzer."
"The iTRAQ reagents label all the peptides in four different samples, which means we do 10,000 or more MS/MS mass spectra per experiment."
"Using the iTRAQ reagents with the 4700 Proteomics Analyzer has been a turning point in our research because it means we can perform quantitative analyses of the synapse proteome on a routine basis."
CRISPR Reveals New Targets for Promising Cancer DrugsNews
Novel screening method identifies new drug targets that could potentially enhance the effectiveness of PD-1 checkpoint inhibitors, a promising new class of cancer immunotherapy.READ MORE
Study Indicates 75% of Human Genome is Non-functionalNews
An evolutionary biologist at the University of Houston has published new calculations that indicate no more than 25 percent of the human genome is functional.READ MORE
Molecular Mechanism That Enable Neuronal Connections to Change With Development DiscoveredNews
Researchers from King's College London have discovered a molecular mechanism that enables neuronal connections to change through experience, thus fuelling learning and memory formation.READ MORE
Comments | 0 ADD COMMENT
EMBL Course: Next Generation Sequencing: RNA Sequencing Library Preparation
Apr 23 - Apr 27, 2018
EMBO Practical Course: Microbial Metagenomics: A 360º Approach
Apr 23 - Apr 30, 2018
EMBL Course: Next Generation Sequencing: Whole Genome Sequencing Library Preparation
Apr 16 - Apr 20, 2018