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Enhancing On-line Biological Sample Processing

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Biological sample pretreatment is an important step in biological sample analysis. Due to the diversity of biological matrices, the analysis of target substances in these samples presents significant challenges to sample processing. To meet these emerging demands on biopharmaceutical analysis, this paper summarizes several new techniques of on-line biological sample processing: solid phase extraction, solid phase micro-extraction, column switching, limited intake filler, molecularly imprinted solid phase extraction, tubular column, and micro-dialysis. We describe new developments, principles, and characteristics of these techniques, and the application of liquid chromatography-mass spectrometry (LC-MS) in biopharmaceutical analysis with these new techniques in on-line biological sample processing.


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Peng, J., Tang, F., Zhou, R., Xie, X., Li, S., Yu, P., & Mu, L. (2016). New techniques of on-line biological sample processing and their application in the field of biopharmaceutical analysis. Acta pharmaceutica Sinica. B., 6(6), 540–551. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/27818920