Gastric Proton Pump Structure Determined
The gastric proton pump, also known as H+,K+-ATPase, is expressed on the surface of the stomach to secrete the gastric acid indispensable for digestion of proteins in food. However, too much acid secretion induces ulcers. The gastric proton pump uptakes protons from the neutral cytoplasm (pH 7) to the acidic milieu of the stomach (pH 1) driven by ATP as an energy source. Thus, it is an important drug target for acid-related diseases. Credit: Kazuhiro Abe.
The highly acidic environment in the stomach is essential for digestion. Furthermore, it acts as an important barrier to pathogens invading via the oral route. However, excessive stomach acidification leads to ulcers. Although this is not a life-threatening condition, it can considerably impair the health of affected individuals. Acid suppression in combination with antibiotics is the recognized treatment to eradicate the bacteria Helicobacter pylori, a risk factor for gastric cancer. This environment of pH1 is regulated by the gastric H+ (hydrogen ions), K+ (potassium ions)-ATPase, a class of enzymes that catalyze H+ transport from neutral cytosolic solution (pH 7) to the acidic gastric lumen (pH 1) fueled by cellular energy source ATP. Hence, gastric H+, K+-ATPase are prominent target for drugs that treat excess stomach acid secretion.
The main research question in this area of study is how such a highly acidic environment can be attained in the stomach. To address this question, one must first elucidate the structure of H+, K+-ATPase, which is exactly what a team of Nagoya University-centered researchers set out to do - and achieved. The team recently published their findings in Nature.
"In our study, we used X-ray crystallography to determine the structures of gastric H+, K+-ATPase bound to two proton pump inhibitors, vonoprazan and SCH28080," study first author Kazuhiro Abe explains. "This information is important for both the refinement of existing drugs and the discovery of new drugs."
X-ray crystallography is a technique that uses x-ray diffraction patterns to determine high-resolution, three-dimensional structures of molecules such as proteins, small organic molecules, and materials.
The team successfully solved the crystal structure of H+, K+-ATPase in complex with either vonoprazan or SCH28080 to a resolution of 2.8 Å - one that is high enough to reveal that the drugs partially overlapped but had clearly distinct binding modes in their binding sites found in the middle of a conduit running from the gastric lumen to the cation-binding site.
"The crystal structures suggest that the tight configuration at the cation-binding site lowers the pKa (a measure of acid strength) value of glutamic acid at residue 820 sufficiently to enable the release of a proton even into the pH 1 environment of the stomach (Fig.3)," senior author Yoshinori Fujiyoshi says. "These structures define the molecular interaction between P-CABs (K+-competitive acid blockers) and H+, K+-ATPase, and reveal how H+, K+-ATPase expels H+ into the stomach even at pH1. Such information will contribute significantly to the knowledge base for drug discovery for conditions related to excessive stomach acidification."
This article has been republished from materials provided by Nagoya University. Note: material may have been edited for length and content. For further information, please contact the cited source.
Crystal structures of the gastric proton pump. Kazuhiro Abe, Katsumasa Irie, Hanayo Nakanishi, Hiroshi Suzuki & Yoshinori Fujiyoshi, Nature, DOI: 10.1038/s41586-018-0003-8.