Investigating Leukaemias Using Proteomics Technologies from Applied Biosystems
News Apr 24, 2006
Researchers at the University of Manchester are using an Applied Biosystems QSTAR® XL Hybrid LC/MS/MS System and a 4000 Q TRAP® LC/MS/MS System with iTRAQ™ reagents to systematically analyse the proteomes of normal and malignant stem cells, such as those involved in cancers like chronic myeloid leukaemia.
"Proteomic analysis provides us with extra information that transcriptomic analysis cannot achieve, such as post-translational regulatory mechanisms that can affect the amount and function of proteins within the cell," explained Professor Tony Whetton, Professor of Cancer Cell Biology at the University.
"One of the exciting things about the iTRAQ labelling reagents is that even with tiny amounts of protein, as little as 40 mg, we can get relative quantification of 800-900 proteins from stem cells."
"Also, phosphorylation and other protein post-translational modifications can occur, which are functionally pivotal to the action of important leukaemia causing genes, including BCR/ABL and NPM/ALK."
"With our approach we can identify and characterise these phosphorylation sites on key specific proteins, via MS-based phosphopeptide analyses."
"The QSTAR System's sensitivity and resolution are critical for relative quantification mass spectrometry, and being able to do MRM on the Q TRAP System is essential for us to perform phosphopeptide analyses with high sensitivity."
With Professor Caroline Dive, Professor Whetton is additionally acquiring an Applied Biosystems 4800 MALDI TOF/TOF™ Analyzer and another 4000 Q TRAP System for the definition of biomarkers in serum or plasma samples relating to prognosis, diagnosis and efficacy in cancer treatment.
"The complexity of serum samples demands the highest level of separation and analytical technology to find such biomarkers," said Professor Whetton.
"Using these instruments provides us with great opportunities for discovering and quantifying peptides and proteins imbued with diagnostic information."
Using Milk Protein to 3D-Imprint Muscle and Bone CellsNews
Researchers from the University of Canterbury are replicating a 3D imprint of cells onto films made of milk protein. The films then gradually degrade, leaving the grown tissue behind.READ MORE
Comments | 0 ADD COMMENT
EMBO Workshop: Integrating Systems Biology: From Networks to Mechanisms to Models
Apr 15 - Apr 17, 2018
EMBL Course: Transgenic Animals - Micromanipulation Techniques
Apr 10 - Apr 11, 2018
EMBO Practical Course: Extracellular Vesicles: From Biology to Biomedical Applications
Apr 09 - Apr 13, 2018
EMBL Course: Introduction to Metabolomics Analysis
Mar 20 - Mar 23, 2018
EMBO | EMBL Symposium: Tissue Self-Organisation: Challenging the Systems
Mar 11 - Mar 14, 2018