LEAP is a provider of automated Hydrogen Deuterium Exchange (HDX) workstations that allow for labeling, quenching and injection of protein samples under cold conditions. Antec is a provider of the ROXY electrochemical system, providing fast and efficient electrochemical reduction of disulfide bonds in proteins/peptides. The integration the ROXY EC system into LEAP’s PAL HDX workstation will assure a fully automated HDX/EC/MS workflow which can be used with every mass spectrometer.
The full integration enables reducing disulfide bonds in proteins/peptides in only a fraction of the time compared with the use of reducing agents (TCEP). Large and highly disulfide-stabilized proteins (e.g., cystine knots) previously difficult to reduce chemically are now accessible for HDX, resulting in an overall increased sequence coverage. Jean-Pierre Chervet (CEO Antec) comments: we are excited to collaborate with LEAP the world leader in HDX automation and to bring HDX/MS to a new level of customer satisfaction by integrating the disulfide bond reduction.
LEAP and Antec are proud to be providing an improved customer solution of HDX/EC/MS integration.