We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Measurement of Pore Diffusivity in Separation Media for High Performance Liquid Chromatography
News

Measurement of Pore Diffusivity in Separation Media for High Performance Liquid Chromatography

Measurement of Pore Diffusivity in Separation Media for High Performance Liquid Chromatography
News

Measurement of Pore Diffusivity in Separation Media for High Performance Liquid Chromatography

Read time:
 

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "Measurement of Pore Diffusivity in Separation Media for High Performance Liquid Chromatography"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Abstract
It was demonstrated that the peak parking-moment analysis (PP-MA) method could be used as an alternative for the measurement of pore diffusivity (D(p)) in porous packing materials for HPLC. The value of D(p) of uracil was measured by the PP-MA method under non-retained conditions in the RPLC systems using C18-silica gel particles and C18-silica monolith as the stationary phase and 70 vol% methanol as the mobile phase. The value of D(p) was compared with that derived by the pulse response-moment analysis (PR-MA) method under the same conditions. The D(p) values of uracil derived by the PP-MA and PR-MA methods were almost in agreement with each other. Because the PP-MA and PR-MA methods have complementary characteristics as experimental methods for studying mass transfer kinetics, it is expected that the combination of the two methods would lead to an effective strategy for the measurement of D(p) in porous separation media for HPLC.

The article is published online in the journal Analytical Sciences and is free to access.

Advertisement