Measurement of Pore Diffusivity in Separation Media for High Performance Liquid Chromatography
News Apr 29, 2013
It was demonstrated that the peak parking-moment analysis (PP-MA) method could be used as an alternative for the measurement of pore diffusivity (D(p)) in porous packing materials for HPLC. The value of D(p) of uracil was measured by the PP-MA method under non-retained conditions in the RPLC systems using C18-silica gel particles and C18-silica monolith as the stationary phase and 70 vol% methanol as the mobile phase. The value of D(p) was compared with that derived by the pulse response-moment analysis (PR-MA) method under the same conditions. The D(p) values of uracil derived by the PP-MA and PR-MA methods were almost in agreement with each other. Because the PP-MA and PR-MA methods have complementary characteristics as experimental methods for studying mass transfer kinetics, it is expected that the combination of the two methods would lead to an effective strategy for the measurement of D(p) in porous separation media for HPLC.
The article is published online in the journal Analytical Sciences and is free to access.
Thermo, SRI International Collaborate to Enhance Small Molecule ResearchNews
Thermo set to collaborate with SRI International to combine results of Orbitrap LC/MS experiments with organism-specific metabolic pathway and genome data to improve small molecule research and analysis.READ MORE
One-step Protein Purification Achieves High Yields, Purity and ActivityNews
A novel method to improve the high-yield, high-purity, high-activity purification of complex proteins by 10- to 500-fold has been developed at the University of Alabama at BirminghamREAD MORE
A Rapid, Reliable Way to Determine the Freshness of FruitNews
Scientists have found a quick and reliable way to test the freshness of the fruit in your grocer’s produce section.
Comments | 0 ADD COMMENT
18th International Conference and Exhibition on Analytical & Bioanalytical Chromatographic Techniques
Nov 02 - Nov 03, 2017
8th Edition of International Conference on Mass Spectromerty
Mar 12 - Mar 13, 2018