Miniaturized Mass-Spectrometry-Based Analysis System for Examination of Conditioned Cell Culture Media
News Nov 05, 2012
We present a fully automated setup for performing in-line mass spectrometry (MS) analysis of conditioned media in cell cultures, in particular focusing on the peptides therein. The goal is to assess peptides secreted by cells in different culture conditions. The developed system is compatible with MS as analytical technique, as this is one of the most powerful analysis methods for peptide detection and identification. Proof of concept was achieved using the well-known mating-factor signaling in baker's yeast, Saccharomyces cerevisiae. Our concept system holds 1 mL of cell culture medium and allows maintaining a yeast culture for, at least, 40 hours with continuous supernatant extraction (and medium replenishing). The device's small dimensions result in reduced costs for reagents and open perspectives towards full integration on-chip. Experimental data that can be obtained are time-resolved peptide profiles in a yeast culture, including information about the appearance of mating-factor-related peptides. We emphasize that the system operates without any manual intervention or pipetting steps, which allows for an improved overall sensitivity compared to non-automated alternatives. MS data confirmed previously reported aspects of the physiology of the yeast-mating process. Moreover, matingfactor breakdown products (as well as evidence for a potentially responsible protease) were found.
The article is published online in the International Journal of Proteomics and is free to access.
Changes Identified in Body Cells During Type2 Diabetes OnsetNews
Researchers have found fresh evidence to explain the processes that occur in the body’s cells leading to the onset of type2 diabetes. Utilising metabolomics, results showed that amino acids play a contributory role in the onset of type2 diabetes in some younger and older patients, as the metabolism of specific amino acids is adversely affected.
Blood Sample Detection Method for Multiple Sclerosis DiscoveredNews
A method for quickly detecting signs of multiple sclerosis has been developed by a University of Huddersfield research team. The process will avoid the invasive, painful process of collecting fluid from the brain and spineREAD MORE
Honey Bee Gut Metabolome Reveals How They Cope With a Pollen Rich DietNews
The honey bee gut is colonized by specialized bacteria that help digest components of the floral pollen diet and produce molecules that likely promote bee health. Researchers studied the metabolome from the bee gut and have uncovered which bacterial species perform which specific digestive functions.