Pressure BioSciences, Inc. has announced that it has developed a method for extraction of proteins from lipid-rich samples, including adipose and brain tissues, organelles, and membrane preparations.
This patent-pending method combines the use of the Company’s pressure cycling technology (PCT) with certain organic solvents. This method can offer an advantage over existing techniques in that it enables protein extraction from lipid-rich samples without the use of detergents. This is important because detergents can cause serious problems in the subsequent analysis of these samples.
The Company plans to discuss its findings at a scientific poster session of the American Society of Mass Spectrometry (ASMS) Conference on Mass Spectrometry being held in Indianapolis, Indiana.
Proteomic analysis of lipid-rich tissue is used in studies of type II diabetes, obesity, certain cancers, ALS, and a number of other serious human disorders. Current detergent-based methods for the extraction of proteins from lipid-rich samples can generate variable results, increasing the cost and processing time for the extraction and compromising the quality and quantity of the recovered proteins. Any method that can avoid the use of detergents may offer valuable advantages to researchers studying tissues rich in lipids.
Data to be presented at ASMS Conference on Mass Spectrometry by the Company and its collaborators at the Harvard School of Public Health suggest that costs can be reduced, experimental protocols streamlined, and that the quality and quantity of protein recovery may be improved when lipid-rich tissues are processed with the Company’s PCT-dependent, detergent-free process. The Company believes that these advantages should benefit the thousands of researchers throughout the world who routinely study lipid-rich samples.