Probing the Behaviors of Gold Nanorods in Metastatic Breast Cancer Cells Based on UV-vis-NIR Absorption Spectroscopy
News Apr 05, 2012
In this work, behaviors of positively-charged AuNRs in a highly metastatic tumor cell line MDA-MB-231 are examined based on UV-vis-NIR absorption spectroscopy in combination with inductively coupled plasma mass spectrometry (ICP-MS), transmission electron microscopy (TEM) and dark-field microscopic observation. It is found that characteristic surface plasmon resonance (SPR) peaks of AuNRs can be detected using spectroscopic method within living cells that have taken up AuNRs. The peak area of transverse SPR band is shown to be proportionally related to the amount of AuNRs in the cells determined with ICP-MS, which suggests a facile and real time quantification method for AuNRs in living cells. The shape of longitudinal SPR band in UV-vis-NIR spectrum reflects the aggregation state of AuNRs in the cells during the incubation period, which is proved by TEM and microscopic observations. Experimental results reveal that AuNRs are internalized by the cells rapidly; the accumulation, distribution and aggregation of AuNRs in the cells compartments are time and dose dependent. The established spectroscopic analysis method can not only monitor the behaviors of AuNRs in living cells but may also be helpful in choosing the optimum laser stimulation wavelength for anti-tumor thermotherapy.
The article is published online in PLoS ONE and is free to access.
Portable Analysis and Alloy Fingerprints Reveal Secrets of Picasso BronzesNews
Researchers have completed the first major material survey and study of the Musée national Picasso-Paris’ world-renowned Pablo Picasso bronzes using cutting-edge, portable instruments.
Spintronic Barrier BrokenNews
Spintronic developments, that enhance solid-state devices like hard drives and cell phone components, are increasingly running up against a barrier known as the Slater-Pauling limit, the maximum for how tightly a material can pack its magnetization. Now, a new thin film is poised to break through this decades-old benchmark.READ MORE