Researchers Developed New Technique to Map Elusive Chemical Markers on Proteins
News Jul 07, 2015
Unveiling how the 20,000 or so proteins in the human body work–and malfunction–is the key to understanding much of health and disease.
The new method allows researchers to map critical chemical tags–called phosphates–that bond to amino acids (the building blocks of proteins) in the final stages of creating a protein.
Salk scientists developed antibodies that tag essential chemicals involved in protein action. In this dividing cell, the new antibody (green) marks discrete areas of a critical but elusive chemical reaction that happens when cells divide (DNA in blue and tubulin, a protein needed for cell division, in red).
When a cell produces a protein, molecular synthetic machinery first pieces together the amino acids into a long strand that bends and folds as it lengthens. Enzymes then gather around the structure to make final tweaks–trimming the protein or adding chemical tags. Among these last modifications is phosphorylation–or addition of phosphate–of individual amino acids to change the protein’s function. Until now, pinpointing exactly where (and why) all those phosphates are added has been tricky.
“We know that 9 out of the 20 amino acids can be phosphorylated, but we know very little about most of them because they’re so hard to study,” says Tony Hunter, American Cancer Society Professor, holder of the Dulbecco Chair in the Salk’s Molecular and Cell Biology Laboratory and senior author of the new paper.
When phosphate is added to three particular amino acids–serine, threonine and tyrosine–it forms a strong chemical bond. Researchers can easily identify the location of these phosphates. But when the other six amino acids are phosphorylated, the phosphate is only loosely attached to each amino acid, causing problems for researchers trying to glean what happens in these cases. One of these amino acids, called histidine (or phosphohistidine once a phosphate is added), has been particularly tough to study.
“With those strong phosphorylation events, you can label cells, isolate proteins and analyze the proteins in various ways to find out where the phosphates are,” says Hunter. “You can’t do that with phosphohistidine because it’s so unstable it falls apart as you’re trying to isolate the proteins.”
Hunter and his collaborators realized that to study this unstable interaction, they would have to use a trick to make a stronger bond between phosphate and histidine. So they used a special kind of modified phosphate, called phosphonate, engineered to bind more tightly to either of the two spots on a histidine amino acid where phosphate can be added. Then, they developed antibodies that specifically recognize these stable phosphohistidine analogues, but also detect authentic phosphohistidine in proteins.
To test these new tools, the team added their phosphohistidine antibodies to a collection of different mammalian cells grown on slides and observed where in the cell the antibodies bound, which indicates parts of cells that have high levels of proteins with phosphohistidines.
“The thing that surprised us most is that when we stained the cells with the new antibodies, we saw discrete areas within the cells that had high levels of histidine phosphorylation, particularly when they were undergoing mitosis, the stage at which cells divide into two daughter cells,” says Hunter. They don’t yet know exactly why that is, but plan to continue to explore these results as well as detect the phosphorylation of other amino acids.
The team expects these antibody tools to be useful to other labs aiming to determine whether proteins of interest have any phosphohistidines.
The new method is “fairly easy for any lab to use,” Hunter says. “It doesn’t require a special instrument or anything, so I think it may be fairly quickly adopted.”
Catalyst Can Degrade Alzheimer's-Related Amyloid Peptide Under Near-Infrared LightNews
A new, biocompatible photooxygenation catalyst that can selectively oxygenate and degrade the pathogenic aggregation of Alzheimer's disease (AD)-related amyloid-β peptide (Αβ) under near-infrared (NIR) light irradiation has been developed.READ MORE
Mediterranean Diet Could Improve Postmenopausal HealthNews
The heart-healthy Mediterranean diet also appears to be good for an older woman's bones and muscles, a new study of postmenopausal women in Brazil finds.READ MORE
Comments | 0 ADD COMMENT
World Congress on Pathology and Laboratory Medicine
Sep 10 - Sep 11, 2018
World Congress on Advances in Addiction Science and Medicine
Sep 24 - Sep 25, 2018
International Conference on Molecular Biology and Stem Cells
Aug 13 - Aug 15, 2018
World Congress on Plant Science and Molecular Biology
Sep 12 - Sep 13, 2018