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Structural Blueprints of Lnt Enzyme Revealed

News   Aug 24, 2017 | Original Story from Trinity College Dublin

 
Structural Blueprints of Lnt Enzyme Revealed

(a) View from the membrane plane. The protein has two domains, a membrane domain and a periplasmic nitrilase-like domain. The structure is shown in cartoon representation and rainbow colour coded from N (blue) to C terminus (red). The catalytic cysteine Cys387 side chain is shown in sphere representation (carbon, magenta; sulfur, yellow). The magenta arrow indicates the proposed substrate entry portal and identifies what is referred to as the front of the enzyme. Approximate location of the membrane boundaries are shown as horizontal lines. Cys387 sits ∼13 Å above the bulk membrane surface. (b) Schematic representation of the secondary structure elements in the LntEco structure. Colour coding follows that used in a. Credit: Professor Martin Caffrey, Trinity College Dubin.

 
 
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