The experiment assessed the performance of Wyatt Technology’s Calypso™ automated composition gradient light scattering system for the evaluation of the inhibition efficacy of AEBSF, a small-molecule, irreversible inhibitor, on the self-association of the digestive enzyme a-chymotrypsin. The Calypso is able to perform accurate and rapid analysis of inhibition kinetics of protein-protein binding as well as equilibrium association properties. The new application note, entitled “Inhibition Kinetics of Protein-Protein Binding with Calypso”, is available to download free-of-charge via www.wyatt.com.
CG-MALS is a powerful, non-destructive method for efficient characterization of macromolecular interactions without labeling, immobilization or additional biochemical substrates. It employs a series of samples of different composition or concentration in order to characterize reversible self- and hetero-association of proteins, reaction rates and affinities of aggregation or dissociation and non-specific, repulsive or attractive macromolecular interactions. The Calypso offers superior analytical capabilities, simplifying and automating CG-MALS measurements. The system's automation enhances productivity by improving repeatability and reliability, while minimizing time and effort. The Calypso offers many advantages over other biophysical techniques for characterizing protein interactions, providing fast and accurate results.