Wyatt Technology Uses Composition Gradient Multi-Angle Light Scattering for Characterizing Protein Inhibition in Solution Without Labeling or Immobilization
News Sep 23, 2009
The experiment assessed the performance of Wyatt Technology’s Calypso™ automated composition gradient light scattering system for the evaluation of the inhibition efficacy of AEBSF, a small-molecule, irreversible inhibitor, on the self-association of the digestive enzyme a-chymotrypsin. The Calypso is able to perform accurate and rapid analysis of inhibition kinetics of protein-protein binding as well as equilibrium association properties. The new application note, entitled “Inhibition Kinetics of Protein-Protein Binding with Calypso”, is available to download free-of-charge via www.wyatt.com.
CG-MALS is a powerful, non-destructive method for efficient characterization of macromolecular interactions without labeling, immobilization or additional biochemical substrates. It employs a series of samples of different composition or concentration in order to characterize reversible self- and hetero-association of proteins, reaction rates and affinities of aggregation or dissociation and non-specific, repulsive or attractive macromolecular interactions. The Calypso offers superior analytical capabilities, simplifying and automating CG-MALS measurements. The system's automation enhances productivity by improving repeatability and reliability, while minimizing time and effort. The Calypso offers many advantages over other biophysical techniques for characterizing protein interactions, providing fast and accurate results.
PhoreMost Completes $15M (£11M) Series-A Round to Enter Drug DiscoveryNews
Investment to fund expansion of operations and progression of drug target pipeline.READ MORE
Targeting a Leaky Protein that Causes ParalysisNews
A rare genetic disorder in which people are overcome suddenly with profound muscle weakness is caused by a hole in a membrane protein that allows sodium ions to leak across cell membranes. Compounds containing a chemical group called guanidinium can block the pore created by the mutation and stop the sodium leak without altering the voltage sensor’s ability to function.READ MORE
Computation and Chemistry Combine to Create World-First Auxetic ProteinNews
A team of chemists at the University of California, San Diego (UCSD) has now designed a two-dimensional protein crystal that toggles between states of varying porosity and density. This is a first in biomolecular design that combined experimental studies with computation done on supercomputers. The research, published in April 2018 in Nature Chemistry, could help create new materials for renewable energy, medicine, water purification, and more.
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