5 Steps to Multiplexed Fluorescent Western Blotting

Tumor associated macrophages (TAMs) can facilitate the proliferation of tumor cells and are able to suppress anti-tumor immune responses within the tumor microenvironment, making them a highly attractive target for the development of innovative cancer immunotherapies.

Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers

Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing

All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.

Download this poster to discover the five key steps in applying flow cytometry for rare events analysis, helping you to ensure that your results and data are accurate, thorough and reproducible.