5 Steps to Multiplexed Fluorescent Western Blotting
Poster Aug 30, 2019
Thermo Fisher Scientific
Thermo Fisher Scientific offer you their expert knowledge in this insightful poster, which provides the reader with all the information they need to successfully and quickly analyze their proteins via western blotting.
This poster will offer advice on troubleshooting major issues with your blots, plus take you through the stages of analysis including:
- Sample preparation
- Separating proteins by gel electrophoresis
- Transfering proteins to membrane
- Detecting proteins with labeled antibodies
- Imaging and quantitating your proteins
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.