A Novel Instrument for High-throughput, High-speed Cell Counting, Concentration, and Viability
Poster Jun 08, 2020
Jordan Bell, Suzanne M Purseglove, Leo Li-Ying Chan, and Dmitry Kuksin
The monotonous and time-consuming task of manually counting cells on a hemocytometer has been greatly alleviated in recent years with the advent of various automated cell-counting instruments. Nevertheless, the measurement of cell concentration and viability for many samples remains a common bottleneck in large scale experiments.
Download this poster to discover a system that has addressed these challenges, with the capability to:
- Count 24 samples in as little as 48 seconds
- Use bright field and fluorescence counting options
- Increase both accuracy and throughput
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE