Automated, Low Volume Dynamic Light Scattering Technology to Accelerate Protein Crystallization
Poster Nov 02, 2006
Kevin Jackson and Robert Collins
The quest for solving protein structure largely relies upon X-Ray diffraction, a method requiring crystalline forms of the target protein. Among the many steps comprising structure determination, the process of protein crystallization represents one of the most significant, time-consuming challenges. A new low sample volume, automated dynamic light scattering (DLS) technology has been developed – the DynaPro Plate Reader.
The DynaPro Plate Reader can measure protein samples less than 5 microliters in volume, at less than 1 mg/mL concentrations in 1536 well-plate formats. By analyzing 100’s of samples per day, in an unattended fashion, the DynaPro Plate Reader improves the productivity of vital dynamic light scattering applications in the protein crystallization process: screening proteins for aggregation prior to crystallization trials and solubility screening. More conditions can be explored in less time with less sample, leading to the successful optimization of the protein and/or crystallization conditions.
Measuring the 2nd Virial Coeffient and Molecular Density of Proteins to Improve CrystallizationPoster
The second virial coefficient of Human Serum Albumin (HSA) was measured using a SEC-MALS 20 detector connected to a Refractive Index concentration detector and an online differential viscometer. Two different buffer conditions, Phosphate buffer pH 7.4 and Citrate buffer pH 4.1 were used, which revealed interesting and contrasting dataREAD MORE
"In-vitro studies on hydroxamic acid- CT DNA binding"Poster
In vitro studies of five the interaction between five different derivatives of hydroxamic acids, N-phenyl 2,4dichloro phenoxybutyro, N-m-tolyl 2,4 dichlorophenoxyglutero, N-m-tolyl-4-chlorophenoxyaceto, N-m-chloro-phenyl-tertiarybutylbenzo and N-p-tolyl-iso-valero hydroxamic acids and calf thymus DNA was investigated under simulated physiological condition.READ MORE
Rapid Quantification of Proteins in Complex Matrices using the DeNovix DS11 Microvolume SpectrophotometerPoster
In this poster, we will introduce the DeNovix DS-11 as the next generation in microvolume spectrophotometry.READ MORE