C1 Complement mediates human cord blood serum derived APP α-secretase cleavage activity in vitro
Poster May 08, 2018
Anran Fan, Catherine Wang, Ahsan Habib, Darrell Sawmiller, Huayan Hou,1Manasa Kanithi, Dan Zi, Zhixu He, Paul R. Sanberg and Jun Tan.
Alzheimer’s disease (AD) is the leading cause of dementia in the elderly. In healthy individual amyloid precursor protein (APP) is cleaved by α-secretase generating sAPPα. However, in the neurodegenerative environment of AD patients, Aß peptides of either 40 or 42 residues are generated by increased beta and gamma secretase activity. Human umbilical cord blood cells (UCBC) have proven useful as potential immunomodulatory therapies in various models of neurodegenerative diseases. Our study investigated the impact of UCBS on modulation of sAPPα production. Heat-activated UCBS has significantly promoted sAPPα production indicating presence of heat sensitive α-secretase in CBS. Using LC-MS/MS, We identified the subunits of C1 complex (C1q, C1r and C1s) and α-2-macroglobulin showed significantly greater levels in αCBSF compared with AgBSF. Specifically, C1 markedly increased sAPPα and αCTF production, whereas C1q alone only minimally increased and C3 did not increase sAPPα production in the absence of sera. Furthermore, C1q markedly increased sAPPα and αCTF, while decreasing Aß, in CHO/APPwt cells cultured in the presence of whole sera. These results confirm that APP α-secretase activity in human blood serum is mediated by C1 opening a potential modality of therapeutic for the future of AD.
Treatment Options for Chronic Parvovirus Viremia in Pediatric Heart Transplant Patients in a Tertiary Care CenterPoster
This abstract discusses three cases of pediatric heart transplant patients who suffered from parvovirus (B19) infection. Of these patients, two ( B & C) responded well to standard intravenous Ig therapy. Patient A however, did not respond to standard treatment and was begun on subcutaneous Ig, which effectively diminished his viral load. Thus, subcutaneous Ig infusions might serve as a second line treatment for transplant patients with parvovirus who do not respond well to the standard approach.READ MORE
T-helper cell phenotype expression in cutaneous lesions of angioimmunoblastic T-cell lymphomaPoster
Angioimmunoblastic T-cell lymphoma (AITL) is a common type of peripheral T-cell lymphoma. AITL can be missed until lymphadenopathy develops in patients initially presenting with skin lesions, as skin biopsy may lack conclusive findings. Our case highlights the extranodal presentation of AITL with cutaneous lesions displaying the TFH phenotype.READ MORE
The Utility of Pediatric Asthma Severity Scores in Disposition Decisions for Status AsthmaticusPoster
We aimed to determine if the Pediatric Asthma Severity Score (PASS) predicts a patient's appropriate level of care in the inpatient setting, with special attention to patients who required rapid transfer to another level of care after initial disposition and/or hospital readmission.READ MORE
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9th International Conference on Mass Spectrometry and Chromatography
Sep 21 - Sep 22, 2018