Determination of Water-and Fat-Soluble Vitamins in Gummies by Reversed-Phase Liquid Chromatography
Poster Mar 15, 2017
Changling Qiu & Douglas E. Raynie
Vitamins are essential substances for human health and growth. Storage, aging, and processing of foods may cause vitamin loss. There is a need to develop a fast and accurate analytical method for the determination of vitamins in foods.
The determination of vitamins in sugar-based matrix is usually a challenging task due to the complexity of the matrix and instability of vitamins. Gummies are sugar-based candies which are fortified with selected vitamins.
Until now, no method has been proposed for the determination of vitamins in gummies. In this work, the determination of six water-soluble vitamins and three fat-soluble vitamins is studied by reverse phase liquid chromatograph(RP-LC) with ultra-violet detector at different wavelengths, on a C-8 column using phosphate buffer and acetonitrile as mobile phase.
Partial characterization of Plasmodium falciparum protein kinase ABCK2 (PfABCK2)Poster
PfABCk2 gene is ligated into pET21a+ vector with His-tag at C-terminus and transformed into BL-21 (DE3) competent cells that are verified through Miniprep and DNA sequencing. Furthermore, this gene construct is utilized to heterologous express this protein with IPTG and afterwards purified the recombinant protein kinase using nickel affinity chromatography as shown on 10% SDS-PAGE with the expected 36 kDa protein band.READ MORE
From Farm to Landfill: Results of a Campus-Community Awareness Event to Promote Sustainable Food SystemsPoster
In April 2017, a campus-community event (N=42 participants) was held to promote sustainable food systems by educating participants about concepts ranging from food production to nutrition and food waste management. This poster discusses the resulting increased awareness and knowledge of a our local food system among participants, which can be fostered through educational events that combine opportunities for research, teaching, and practice.READ MORE
Environmental Exposures and Inflammatory Bowel Disease in ChildhoodPoster
Inflammatory bowel diseases (IBD) often present in adolescence and affect >1.4 million persons in the US. Environmental exposures, notably air pollution and weather, have been suggested to contribute to IBD disease activity. Our goal was to evaluate relationships between environmental exposures and the prevalence and disease activity of pediatric IBD using novel data sources that take into account geographic location.READ MORE