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Determination of Water-and Fat-Soluble Vitamins in Gummies by Reversed-Phase Liquid Chromatography

Vitamins are essential substances for human health and growth. Storage, aging, and processing of foods may cause vitamin loss. There is a need to develop a fast and accurate analytical method for the determination of vitamins in foods.

The determination of vitamins in sugar-based matrix is usually a challenging task due to the complexity of the matrix and instability of vitamins. Gummies are sugar-based candies which are fortified with selected vitamins.

Until now, no method has been proposed for the determination of vitamins in gummies. In this work, the determination of six water-soluble vitamins and three fat-soluble vitamins is studied by reverse phase liquid chromatograph(RP-LC) with ultra-violet detector at different wavelengths, on a C-8 column using phosphate buffer and acetonitrile as mobile phase.