Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSI
Poster Nov 21, 2017
E. E. L. Lewis, M. R. T. Barrett, D. Caballero-Lima, R. A. Bojar and M. R. Clench
Infections are known to have a detrimental effect on wound healing leading to chronic would formation. Chronic wounds are becoming a burden for western societies due to increased incidences of obesity, diabetes and ageing populations. This health crisis has a vast effect on wound care management resources and budgets worldwide. Effective diagnosis and wound management has been shown to reduce costs and chronic wound formation. Therefore it is vital to understand the molecular mechanisms involved in the wound healing process. Labskin is a human living skin equivalent model (LSE), which when used in combination with Mass Spectrometry Imaging (MSI) is able to assess biological changes within non-infected and infected wounds to identify biomarkers associated with wound healing. Here we report the development of a novel incision method for wounding LSE: infection of these wounds and their study by MALDI-MSI.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE