Extraction of Analytes of Toxicolgical Interest from Plasma with Enhanced Matrix Removal-Lipid Material
Poster Mar 09, 2017
Joan Stevens, Derick Lucas, Limian Zhao
Matrix interferences and ion suppression are two common analytical issues often encountered when using chromatographic techniques in drug analysis. Diluting the sample prior to analysis especially for LC/MS analysis is a common technique however it does not reduce matrix resulting in ion-suppression. Lack of sample preparation will reduce the method robustness, create chromatographic anomalies and increase instrument maintenance. Lipids are the major source of matrix interferences in plasma and will build up on the analytical column and cause ion-suppression.
Removing lipids from a biological sample not only increases the robustness of the analytical method but improves recoveries and limits of quantitation/detection. Protein precipitation is a simple sample preparation technique that removes the proteins but the lipids remain in the sample. Solid phase extraction can produce a very clean sample removing proteins and lipids, however it requires technical expertise and additional laboratory equipment. Implementing protein precipitation with a novel enhanced matrix removal-lipid material offers the cleanliness of a solid phase extraction method with the simplicity of protein precipitation.
Using a mixture of controlled substances and drugs of abuse at low ng/mL levels we will demonstrate the simple yet effective sample preparation technique incorporating EMR-Lipid.
Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent ModelPoster
Untargeted proteomics analysis showed that human skin and a commercially available living skin equivalent model exhibit a similar distribution of xenobiotic metabolising enzymes. A new technique, substrate based mass spectrometry imaging (SB-MSI) was developed during this study.READ MORE
Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSIPoster
Infections have detrimental effects on wound healing leading to chronic wound formation. The use of labskin,a living skin model, in combination with mass spectrometry imaging, provides a good model for assessing wound healing factors.READ MORE
Overcoming the Challenges of Analysing Ionic Polar Pesticides in FoodPoster
Here, the novel application of the Waters’ Torus DEA column is presented, showing the improved performance of a UPLC-MS/MS method for the underivatised
analysis of glyphosate and a selection of other anionic pesticides.