Glycoprotein Labeling and Detection: Novel Click Chemistry-based Applications for gel Electrophoresis, Flow Cytometry and Fluorescence Microscopy
Poster May 22, 2007
We demonstrate highly-selective and sensitive labeling methods for the detection of specific glycoprotein subclasses, including cell surface N- and O-linked glycoproteins and intracellular O-GlcNAc modified proteins, utilizing the copper-catalyzed cycloaddition reaction between azides and alkynes, or click chemistry. The two-step labeling technique involves the incorporation of unnatural azide-modified sugars into protein glycan structures and subsequent ligation with fluorescent or UV-excitable azide-reactive detection probes.
Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)Poster
The method allows for automated determination of total fat content and quantitative analysis of saturated and unsaturated cis- and trans- fatty acids of different chocolate products.READ MORE
Fast Pesticide Residue Analysis Using a Novel Benchtop Time-of-Flight Mass SpectrometerPoster
The objective of the study was to maximize throughput without introducing a significant number of additional chromatographic co-elutions not separable by deconvolution, whilst transitioning from a standard 30 m column to a 15 m column using LECO's software.READ MORE