Next-generation DNase for Ultimate Performance in Molecular Biology Applications
Poster Dec 20, 2019
Darius Kavaliauskas, Viktorija Vitkovskė, Gediminas Alzbutas, Juozas Šiurkus
DNase I is the most widely used enzyme in molecular biology applications such as those for DNA removal from RNA samples and in vitro transcription reactions.
As such, researchers are frequently looking for ways in which they can improve the capabilities of this enzyme and its adaptability to different workflows. This can come in the form of specifically engineered, mutant forms of DNAse I.
Download this poster to discover how a new DNAse I mutant compares to the wildtype in:
- Salt tolerance
- Removal of the DNA template
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE