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Real-Time Multiplex Rt-PCR on Circulating Tumor Cells

Real-Time Multiplex Rt-PCR on Circulating Tumor Cells content piece image
Abstract
Using the CTC kit (CellSearch™), cells that attached to anti-EpCAM Moab were immunomagnetically separated and used for analysis of a selected pilot set of 32 genes by real time RT-PCR. This study shows the feasibility of multiple gene expression analysis on RNA isolated from only one tumor cell. Most importantly, expression analysis of several tumor-specific genes in blood samples containing only 2 tumor cells is already possible.