Screening of ALDH2 Interactome by Combined Immunoprecipitation and Mass Spectrometry
Poster Apr 23, 2018
Irene John, Jutaro Fukumoto, Sahebgowda Patil, Sudarshan Krishnamurthy, Mason Breitzig, Matthew Alleyn, Lakshmi Galam, Ramani Soundarajan, Richard F Lockey,
Kari Basso, Narasaiah Kolliputi.
Mitochondrial aldehyde dehydrogenase 2 (ALDH2) has been shown to protect against oxidative stress in multiple organs. ALDH2 has a role in preventing oxidative stress-induced 4-HNE adduction in multiple organs. As a result, ALDH2 may have an unidentified protein interaction network, which would help elucidate its importance in various cellular functions. Studies show that ALDH2 aids in protecting the lungs against oxidative stress. We are studying ALDH2 in the lung because it has been demonstrated to be critical to the integrity of lung cells and an important shield from damage occurring under oxidative stress.
Since information regarding the ALDH2 interactome is limited, exploring cells which assume different cell profiles, type II AEC and club cell, may give us new insight of ALDH2’s counteractive role against oxidative stress.
Results show that ALDH2 may have an unidentified protein interaction network, which would help elucidate its importance in various cellular functions. The combination of immunoprecipitation and mass spectrometry will give us insights that could not be easily attained otherwise.
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.