Sensitive Identification of Phosphopeptides in Brain Tissue using 2D-NanoLC-ESI-MSn
Poster Aug 25, 2005
Jenny Samskog, Henrik Wadensten, and John Flensburg
IntroductionOne of the most important post-translational modifications is phosphorylation of serine, threonine or tyrosine residues. Detection of phosphorylation sites by mass spectrometry in proteins extracted from biological material is complicated by low abundance, low stoichiometry, and poor ionization of phosphopeptides .
In this work, a biocompatible nano liquid chromatography (LC) system, Ettan™ MDLC, was used for separating tryptic peptides from brain tissue by cation exchange (SCX) to enrich the phosphopeptides followed by reversed-phase chromatography (RPC). The phosphopeptides were detected by neutral loss MS.
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Optimization of a Vacuum Ultraviolet Photoionization source for GC used with a High Resolution TOFMSPoster
-Tune solution allows optimization of ion source parameters for
both proton transfer and direct ionization
-Independent ionization processes exist for M+ and MH+
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-Degree of fragmentation remains relatively constant over a
range of source conditions